Immune and immunohistochemical-related responsive patterns of metoclopramide drug-induced hyperprolactinemia

Nassef M Salem ML El-Desouki N Abdallah F    

Abstract

Immunological and immunohistochemical influences patterns of prolactin (PRL) hypersecretion (hyperprolactinemia, HPRL) have been elucidated in the last decade. Here, we aimed to determine the immune and immunohistochemical-related response patterns of metoclopramide drug (MCP)- induced HPRL on the immune peripheral blood (PB) cells and cytoskeletal vimentin filament protein of adrenal gland in addition to investigate the phenotypic modification of MCP-induced HPRL to the spleen T-lymphocytes surface antigenic markers (CD4 and CD8). For this purpose, elevated PRL levels (HPRL) were established in male Swiss albino mice by intraperitoneally (i.p.) injection of MCP at a dose of 2.2 mg/kg each day for 4 treatment cycles: 2, 4, 7 and 10 weeks. As a result, MCP-induced HPRL resulted in significant increases in total number of WBC and absolute numbers of lymphocytes 2 weeks post MCP treatment. Additionally, phenotypic expression of CD4+ and CD8+ recorded a significant increase, especially at the end of week 2 of MCP exposure. Moreover, there was increment immunoreactivity to vimentin antigen in the adrenal gland of MCP-induced HPRL mice analyzed at the end of week 2 and week 4 from MCP treatment. To conclude, HPRL, induced by MCP, led to immunological perturbations in the immune PB cells and spleen lymphocytes surface markers (CD4+ and CD8+) that were time-dependent. Besides, MCP-induced HPRL caused immunohistochemical alterations in vimentin protein in adrenal gland. Therefore, MCP should not be used for long duration, and must be used under medical supervision.


MS Received Date:   2017-06-22  MS Accepted Date:  2017-08-04  MS Published Date:  2017-08-04
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Nassef M, Salem ML., El-Desouki N., Abdallah F. "Immune and immunohistochemical-related responsive patterns of metoclopramide drug-induced hyperprolactinemia" Journal of Zoology Studies 4.4 (2017): 6-14.
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Nassef, M., Salem, ML., El-Desouki, N., Abdallah, F. (2017). Immune and immunohistochemical-related responsive patterns of metoclopramide drug-induced hyperprolactinemia. Journal of Zoology Studies, 4(4), 6-14.
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Nassef M, Salem ML, El-Desouki N, Abdallah F. Immune and immunohistochemical-related responsive patterns of metoclopramide drug-induced hyperprolactinemia. Journal of Zoology Studies. 2017; 4(4): 6-14.
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