RAPD based genetic diversity of freshwater snail, Pila polita in Bangladesh

Miah MF Leamon AKMA Mahzabin S Rahaman SE    

Abstract

Bangladesh is enriched with fish and other aquatic organisms and the freshwater snail Pila polita is one of them. Like other snails Pila polita is eco-friendly and economically important species, however, study related to genetics particularly diversity and relationships of this snail is very limited. This was the first genetic research of Bangladesh on this species. In this research Random Amplified Polymorphic DNA (RAPD) technique was performed for evaluating the genetic variability among individuals of Pila polita. Seven individuals of Pila polita were analyzed with three random primers such as B 03, C 04 and OPB 12. A total of 11 polymorphic loci were found in experimental samples. In this analysis, highest polymorphism (35.29%) was showed by the primer B03 and lowest polymorphism (25%) was showed by the primer OPB12. The highest and lowest inter-individual pair wise similarity was recorded 4 and 1 respectively. Genetic distance was observed with values highest 0.944 and lowest 0.556 among these experimental individuals. The highest and lowest linkage distance among individuals was observed 17.0 and 5.0 respectively. Nei’s genetic similarity was observed whereas highest and lowest values were recorded 0.500 and 0.111 respectively. In the cluster analysis, it was observed that most of individuals were distinctly related with each other.


MS Received Date:   2014-09-01  MS Accepted Date:  2014-10-19  MS Published Date:  2014-10-28
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Miah MF, Leamon AKMA., Mahzabin S., Rahaman SE. "RAPD based genetic diversity of freshwater snail, Pila polita in Bangladesh" Journal of Zoology Studies 1.5 (2014): 27-32.
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Miah, MF., Leamon, AKMA., Mahzabin, S., Rahaman, SE. (2014). RAPD based genetic diversity of freshwater snail, Pila polita in Bangladesh. Journal of Zoology Studies, 1(5), 27-32.
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Miah MF, Leamon AKMA, Mahzabin S, Rahaman SE. RAPD based genetic diversity of freshwater snail, Pila polita in Bangladesh. Journal of Zoology Studies. 2014; 1(5): 27-32.
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